Enhancer Pathway Enrichment Analysis

Analyze genomic regions for enhancer interactions and pathway enrichment

(for older version click here)

How to Use This Tool

Step 1: Input Your Data

Upload a BED file containing your genomic regions of interest or paste the data directly into the text area. The BED file should contain at minimum three columns: chromosome, start position, and end position.

Step 2: Select Analysis Options

Choose whether to use a custom background or the default background. If using a custom background, upload your background BED file or paste the data.

Step 3: Select Pathway Database

Choose one or more pathway databases for enrichment analysis.

Step 4: Run Analysis

Click the "Run Analysis" button to start the process. Please note:

  • The analysis may take several minutes to complete depending on the size of your input data and selected options.
  • During analysis, a progress bar will indicate the current status.
  • Do not close the browser window or navigate away from this page while the analysis is running.
  • Once complete, results will be displayed below the form, organized into collapsible sections.
Step 5: Interpret Results

Results include:

  • Plots: Visual representations of enrichment analysis results
  • Logs: Detailed information about the analysis process
  • Output Files: Downloadable data files for further analysis

System Prerequisites Check

All required components are available. The system is ready for analysis.

Component Status Details
Upload directory Available
Results directory Available
Python script Available File size: 21436 bytes
GMT directory Available 5 GMT files found
Log directory Available
Enhancer gene file Available File size: 35810623 bytes
Python installation Available Python 3.8.0

Input BED File

Provide a BED file containing genomic regions of interest.

Download Sample BED File Use this sample file to test the tool

No file chosen

BED format (chromosome, start, end)
BED format (chromosome, start, end)

Pathway Databases

Select the pathway databases to include in the enrichment analysis.

Background Options

Choose a background set for statistical comparison.

Analysis Parameters

Configure the parameters for the enrichment analysis.

bp
Maximum distance between enhancers and genes to consider