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About Our Datasets

Our database provides comprehensive datasets of enhancer perturbation experiments, including raw data, processed results, and supplementary materials. All datasets are available in standard formats (xlsx, bed, csv, tsv) and are regularly updated.

Dataset Description Format Size Last Updated Action
Complete Database Full database dump including all enhancer perturbation experiments xlsx 13MB 2024-02-20 Download
Enhancer Coordinates Genomic coordinates of all enhancers xlsx 156 MB 2024-02-20 Download
Enhancer Activity Data Interactive visualization of enhancer activity across tissues HTML/JS 2.1 MB 2024-02-20 View
Enhancer - TF activity data Data on enhancer and transcription factor activity relationships. Directory N/A 2025-09-23 View
Enhancer-gene-list Enhancer gene relationships and annotations bed 45 MB 2024-02-20 Download
Pathway Enrichment Script Stand-alone Python script for enhancer pathway enrichment analysis py 17 KB 2025-05-13 Download
Enhancer Simple info (UCSC bed detail file) Simplified enhancer details in BED format bed 15 MB 2024-02-20
Enhancer Details (UCSC bed detail file) Comprehensive enhancer details in BED format bed 31 MB 2024-02-20 Download
Database TSV Complete database in tab-separated format tsv 48 MB 2024-02-20 Download
Database CSV Complete database in comma-separated format csv 48 MB 2024-02-20 Download
Pathway Enrichment Script

The pathway enrichment Python script allows you to perform enhancer pathway enrichment analysis locally on your own machine. This is the same script that powers our web-based analysis tool.

Requirements:
  • Python 3.6 or higher
  • Required packages: pandas, numpy, pybedtools, scipy, plotly
  • Input BED file with genomic regions
  • GMT files for pathway databases (included in the script package)
Basic Usage:
python pathway_enrichment.py --bed your_input.bed --enhancer enhancer_gene.bed --gmt_dir gmt_directory

For more detailed usage instructions and additional options, download the script and run:

python pathway_enrichment.py --help
Publication Statistics

Total Publications: 35

Publications added to database: Download CSV

Paper_titledoi
CRISPR tiling deletion screens reveal functional enhancers of neuropsychiatric risk genes and allelic compensation effects (ACE) on transcriptionhttps://doi.org/10.1101/2024.10.08.616922
High-resolution interrogation of functional elements in the noncoding genomehttps://doi.org/10.1126/science.aaf7613
CRISPR Screening of Transcribed Super-Enhancers Identifies Drivers of Triple-Negative Breast Cancer Progressionhttps://doi.org/10.1158/0008-5472.can-23-3995
Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAshttps://doi.org/10.1038/s41551-024-01204-8
CRISPR Screening Uncovers a Long-Range Enhancer for ONECUT1 in Pancreatic Differentiation and Links a Diabetes Risk Varianthttps://doi.org/10.1016/j.celrep.2024.114640
A multi-omic dissection of super-enhancer driven oncogenic gene expression programs in ovarian cancerhttps://doi.org/10.1038/s41467-022-31919-8
STAT3-Mediated Promoter-Enhancer Interaction Up-Regulates Inhibitor of DNA Binding 1 (ID1) to Promote Colon Cancer Progressionhttps://doi.org/10.3390/ijms241210041
Enhancer RNA Transcription Is Essential for a Novel CSF1 Enhancer in Triple-Negative Breast Cancerhttps://doi.org/10.3390/cancers14071852
Parallel characterization of cis-regulatory elements for multiple genes using CRISPRpathhttps://doi.org/10.1126/sciadv.abi4360
Androgen Receptor-Activated Enhancers Simultaneously Regulate Oncogene TMPRSS2 and lncRNA PRCAT38 in Prostate Cancerhttps://doi.org/10.3390/cells8080864
Intronic enhancers of the human SNCA gene predominantly regulate its expression in brain in vivohttps://doi.org/10.1126/sciadv.abq6324
Functional CRISPR screen identifies AP1-associated enhancer regulating FOXF1 to modulate oncogene-induced senescenchttps://doi.org/10.1186/s13059-018-1494-1
Massively parallel disruption of enhancers active in human neural stem cellshttps://doi.org/10.1016/j.celrep.2024.113693
Deciphering essential cistromes using genome-wide CRISPR screenshttps://doi.org/10.1073/pnas.1908155116
TALEN-mediated enhancer knockout influences TNFAIP3 gene expression and mimics a molecular phenotype associated with systemic lupus erythematosushttps://doi.org/10.1038/gene.2016.4
Dynamic-network-guided CRISPRi screen identifies CTCF-loop-constrained nonlinear enhancer-gene regulatory activity during cell state transitions//doi.org/10.1038/s41588-023-01450-7
Landscape of enhancer disruption and functional screen in melanoma cellshttps://doi.org/10.1186/s13059-023-03087-5
Systematic mapping of functional enhancer-promoter connections with CRISPR interferencehttps://doi.org/10.1126/science.aag2445
Downregulation of enhancer RNA AC003092.1 is associated with poor prognosis in kidney renal clear cell carcinomahttps://doi.org/10.1038/s41598-024-64431-8
eNEMAL, an enhancer RNA transcribed from a distal MALAT1 enhancer, promotes NEAT1 long isoform expressionhttps://doi.org/10.1371/journal.pone.0251515
Functional roles of enhancer RNAs for oestrogen-dependent transcriptional activationhttps://doi.org/10.1038/nature12210
Enhancer RNA - P2RY2e induced by estrogen promotes malignant behaviors of bladder cancerhttps://doi.org/10.7150/ijbs.27151
Super-enhancer-associated long noncoding RNA AC005592.2 promotes tumor progression by regulating OLFM4 in colorectal cancerhttps://doi.org/10.1186/s12885-021-07900-x
Enhancer RNA-driven looping enhances the transcription of the long noncoding RNA DHRS4-AS1, a controller of the DHRS4 gene clusterhttps://doi.org/10.1038/srep20961
The super-enhancer-driven lncRNA LINC00880 acts as a scaffold between CDK1 and PRDX1 to sustain the malignance of lung adenocarcinomahttps://doi.org/10.1038/s41419-023-06047-w
Super-enhancer-driven lncRNA LIMD1-AS1 activated by CDK7 promotes glioma progressionhttps://doi.org/10.1038/s41419-023-05892-z
Co-activation of super-enhancer-driven CCAT1 by TP63 and SOX2 promotes squamous cancer progressionhttps://doi.org/10.1038/s41467-018-06081-9
Genome-wide profiling of p53-regulated enhancer RNAs uncovers a subset of enhancers controlled by a lncRNAhttps://doi.org/10.1038/ncomms7520
JUN-induced super-enhancer RNA forms R-loop to promote nasopharyngeal carcinoma metastasishttps://doi.org/10.1038/s41419-023-05985-9
The enhancer RNA ARIEL activates the oncogenic transcriptional program in T-cell acute lymphoblastic leukemiahttps://doi.org/10.1182/blood.2018874503
Super Enhancer-Regulated LncRNA LINC01089 Induces Alternative Splicing of DIAPH3 to Drive Hepatocellular Carcinoma Metastasishttps://doi.org/10.1158/0008-5472.CAN-23-0544
Carcinogen-induced super-enhancer RNA promotes nasopharyngeal carcinoma metastasis through NPM1/c-Myc/NDRG1 axishttps://pmc.ncbi.nlm.nih.gov/articles/PMC10492133/
RNAs interact with BRD4 to promote enhanced chromatin engagement and transcription activationhttps://doi.org/10.1038/s41594-018-0102-0
Complementary Alu sequences mediate enhancer?promoter selectivityhttps://doi.org/10.1038/s41586-023-06323-x
The long noncoding RNA SNHG1 regulates colorectal cancer cell growth through interactions with EZH2 and miR-154-5phttps://doi.org/10.1186/s12943-018-0894-x
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